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Batch Enzyme Reactor
Product Code: SUK-SCHOOL-SSLAB-N9000004
Description & Specifications
Batch Enzyme Reactor
Technical Specification
This is a reactor system specially designed to perform batch enzyme
reaction utilizing the glucose isomerization reaction catalyzed by
glucose isomerase. The unit is used to demonstrate batch enzyme kinetics
and enzyme characteristics. The fourth reactor - stirred tank reactor
in series - is mounted on a dedicated unit.
A bench top unit comprising a vacuum formed ABS plastic plinth with
integral electrical console onto which is mounted the stirred reactor
vessel sampling circuit with peristaltic pump, tubular coil heat
exchanger and polarimeter device.
A polarimeter device measuring optical transmission and angle of rotation. Protection devices for all electrical circuits.
Three displays: PID temperature control (reactor temperature), a display
for the angle of rotation, a display for optical transmission or
temperature at polarimeter.
A temperature sensor and heater mounted in the reactor vessel and linked
to a PID controller for accurate reaction temperature control. Sensor
signals are routed to the I/O port for connection to a PC.
A cruciform geometry impeller constructed from stainless steel mesh
retains the immobilized enzyme whilst allowing efficient mixing with the
liquid reactant. The reactors use the saponification reaction between
ethyl acetate and sodium hydroxide. The measurement system relies on the
fact that both glucose and fructose solutions rotate beams of polarised
light, glucose to the right and fructose to the left. This provides a
well understood and safe reaction for the students to investigate the
different reactor types.
It consists of a bench-top unit onto which is mounted a reactor vessel
in which the glucose isomerase-mediated reaction takes place.
The reactor itself is made of clear acrylic, which gives good visibility.
The reaction temperature is maintained using two heaters and a temperature sensor mounted within the reactor.
These are linked to a PID controller, which is programmed to maintain the desired set-point temperature.
Safety interlocks prevent the heaters being activated when there is a low reactor liquid level or when the impeller is inactive.
A continuous sampling loop driven by a peristaltic pump removes liquid
from the reactor and transfers it to a tubular coil heat exchanger where
it is cooled prior to passing through a polar meter where the angle of
rotation of polarised light is measured.
From this angle measurement, the concentration of both glucose reactant and fructose product can be determined.
The impeller is a variable speed type.
This eliminates the need for manual glucose assays.
The polarimetry measurement method allows the progress of the reaction to be monitored on-line.